Rapid embedding of tissues in Lowicryl K4M for immunoelectron microscopy.
نویسندگان
چکیده
منابع مشابه
Lowicryl K4M embedding of brain tissue for immunogold electron microscopy.
We present methods for embedding brain tissue in Lowicryl K4M embedding medium and localizing antigens using postembedding immunogold techniques. After perfusion fixation with 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1 M cacodylate buffer, blocks of rat brain were placed in 2% aqueous uranyl acetate for 1 hour, dehydrated in 50%, 70%, and 95% ethanol, infiltrated with Lowicryl/ethanol m...
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With great interest we read the recent article by Usuda et al. (1), which describes the immunoelectron microscopy of tissue processed by rapid freezing and freeze-substitution without chemical fixatives. In their article, Usuda et al. say that “our result . . . should (sic) be the first demonstration of immunostaining of rapidly frozen tissue processed without chemical fixation.” We would like ...
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Using a low temperature resin Lowicryl K4M and post-embedding immunogold labeling, localization of actin and cytokeratin was examined in hepatocytes and biliary epithelial cells in normal human livers. Preservation of the liver tissues was satisfactory at the electron microscopic level. In immunolabeling of actin, gold particles were mainly found on microvilli and around the junctional complexe...
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The ultrastructural detection and identification of human retroviruses--HTLV (human T-cell lymphotropic virus) and HIV (human immunodeficiency virus)--have become an everyday task for pathologists and virologists as well as for cell and molecular biologists. The development of better and conventionally available immunocytochemical techniques, such as pre- or postembedding immunocytochemical met...
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Recent filoviral outbreaks in animal primates have raised public awareness of the potential for filoviruses to become a public health concern; methods that efficiently identify these viruses are therefore of high priority. An indirect immunoelectron microscopy method, which uses homologous guinea pig polyclonal antiserum, successfully identified Ebola-related (Reston) virus particles in serum a...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1984
ISSN: 0022-1554,1551-5044
DOI: 10.1177/32.11.6436366